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SARS-CoV-2 One-Step RT-PCR Kit, RdRp and N target genes, IVD

Storage Conditions:
-85 °C to -15 °C
NZYtech SARS-CoV-2 One-Step RT-PCR Kit, RdRp and N target genes, IVD provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 genome, through common real-time PCR platforms.
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From €168.00
was €336.00
NZYtech SARS-CoV-2 One-Step RT-PCR Kit, RdRp and N target genes, IVD provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 genome, through common real-time PCR platforms. The virus RNA dependent RNA polymerase (RdRp) and the Nucleocapsid phosphoprotein (N) genes have previously been identified as highly specific markers for SARS-CoV-2. This NZYtech kit targets specific regions in the RdRp and N genes of SARS-CoV-2 genome to provide the highest sensitivity of detection. By using NZYtech SARS-CoV-2 One-Step RT-PCR Kit, RdRp and N target genes, IVD , RNA isolated and purified with a IVD extraction system is retrotranscribed (RT) to cDNA and subsequently amplified by PCR, in a single reaction, using three highly specific primer/probe sets exploiting the so-called TaqMan® principle. During this process, the probes specifically anneal to two regions of the SARS-CoV-2 genome, namely RdRp (within the Orf1ab polyprotein gene) and N genes, in case the sample was extracted from an infected patient. An additional primers/probe set acts as an endogenous internal control to detect nucleic acids of the human ribonuclease P [RNase P gene (RP)], assessing sample quality. In addition, this internal control demonstrates that no reaction inhibition has occurred by PCR inhibitors potentially present in the clinical/environmental samples. To allow identifying the amplification of the three specific targets in a single reaction, SARS-CoV-2 and human RNase P specific probes are differently labelled, with FAM™ and JOE™ reporter dyes, respectively. Note that this panel contains a duplex assay in the same optical FAM™ channel to report an additive performance of the two PCR assays for SARS-CoV-2 detection. In addition, they are provided in optimized concentrations to make sure amplification of human mRNA, even when present at very high concentrations, does not limit the efficiency of the SARS-CoV-2 primers/probe sets. In case the IFU is not available in your official country language, please contact us – info@NZYtech.com so that we can proceed with the translation. Please see languages available below.


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More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application In Vitro Diagnostics (IVD)
Format Liquid
Product Category Predesigned qPCR assays
Compatibility AB™ 7500, AB™ QuantStudio 6 Pro, AB™ StepOnePlus, Bio-Rad® CFX96™, Qiagen Rotor-Gene Q, Roche Life Science LightCycler® 480 II
Marking CE-IVD
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 90 min (from extracted NA to result)
Number of targets Triplex
Internal Control No
Detection Method Probe Based
- NZYSupreme One-step RT-qPCR Master Mix
- SARS-CoV-2(RdRp & N genes)/RP primer/probe Mix (FAM™ and JOE™ labelled)
- SARS-CoV-2(RdRp & N genes)/RP Positive Control (1 x 10^4 copies/μL)
- RNase/DNase free Water
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application In Vitro Diagnostics (IVD)
Format Liquid
Product Category Predesigned qPCR assays
Compatibility AB™ 7500, AB™ QuantStudio 6 Pro, AB™ StepOnePlus, Bio-Rad® CFX96™, Qiagen Rotor-Gene Q, Roche Life Science LightCycler® 480 II
Marking CE-IVD
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 90 min (from extracted NA to result)
Number of targets Triplex
Internal Control No
Detection Method Probe Based
SMART-LAMP: A Smartphone-Operated Handheld Device for Real-Time Colorimetric Point-of-Care Diagnosis of Infectious Diseases via Loop-Mediated Isothermal Amplification. García-Bernalt Diego J, Fernández-Soto P, Márquez-Sánchez S, Santos Santos D, Febrer-Sendra B, Crego-Vicente B, Muñoz-Bellido JL, Belhassen-García M, Corchado Rodríguez JM, Muro A. Biosensors. 2022. https://doi.org/10.3390/bios12060424

Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding. García-Bernalt Diego J, Fernández-Soto P, Muñoz-Bellido JL, Febrer-Sendra B, Crego-Vicente B, Carbonell C, López-Bernús A, Marcos M, Belhassen-García M, Muro A. Journal of Clinical Medicine. 2022. https://doi.org/10.3390/jcm11010158

The Finding of the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) in a Wild Eurasian River Otter (Lutra lutra) Highlights the Need for Viral Surveillance in Wild Mustelids. Padilla-Blanco M, Aguiló-Gisbert J, Rubio V, et al. Frontiers in Veterinary Science. 2022. https://doi.org/10.3389/fvets.2022.826991

Monitoring of the SARS-CoV-2 Omicron BA.1/BA.2 lineage transition in the Swedish population reveals increased viral RNA levels in BA.2 cases. Lentini A, Pereira A, Winqvist O, Reinius B. Med. 2022. https://doi.org/10.1016/j.medj.2022.07.007
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application In Vitro Diagnostics (IVD)
Format Liquid
Product Category Predesigned qPCR assays
Compatibility AB™ 7500, AB™ QuantStudio 6 Pro, AB™ StepOnePlus, Bio-Rad® CFX96™, Qiagen Rotor-Gene Q, Roche Life Science LightCycler® 480 II
Marking CE-IVD
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 90 min (from extracted NA to result)
Number of targets Triplex
Internal Control No
Detection Method Probe Based
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application In Vitro Diagnostics (IVD)
Format Liquid
Product Category Predesigned qPCR assays
Compatibility AB™ 7500, AB™ QuantStudio 6 Pro, AB™ StepOnePlus, Bio-Rad® CFX96™, Qiagen Rotor-Gene Q, Roche Life Science LightCycler® 480 II
Marking CE-IVD
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 90 min (from extracted NA to result)
Number of targets Triplex
Internal Control No
Detection Method Probe Based
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