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T7 RNA Polymerase

Storage Conditions:
-85 °C to -15 °C
T7 RNA Polymerase is a DNA-dependent RNA polymerase with high promoter-specificity. The enzyme only transcribes DNA downstream of a T7 promoter.
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Ready to Ship
Dry Ice
From €49.00
T7 RNA polymerase is a recombinant enzyme purified from Escherichia coli, for the synthesis of high specific-activity RNA probes, biologically active mRNA and antisense RNA. T7 RNA polymerase is DNA-dependent with strict specificity for its own double-stranded promoter that is not efficiently recognized by SP6 or T3 RNA polymerases. T7 RNA polymerase catalyzes the 5’→3’ synthesis of RNA from ribonucleoside triphosphates on single or double-stranded DNA downstream from a T7 promoter. Using circular plasmid DNA as a template will result in heterogeneous transcripts of multiple lengths. T7 RNA polymerase accepts modified nucleotides as substrates for RNA synthesis.
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application In vitro RNA synthesis
Format Enzyme/Protein, Liquid
Product Category RNA Synthesis
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Protocol time Approx. 120 min
– T7 RNA Polymerase (20 U/μL or 200 U/μL)
– Reaction Buffer (10x)
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application In vitro RNA synthesis
Format Enzyme/Protein, Liquid
Product Category RNA Synthesis
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Protocol time Approx. 120 min
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application In vitro RNA synthesis
Format Enzyme/Protein, Liquid
Product Category RNA Synthesis
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Protocol time Approx. 120 min

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