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SKU
MB40901
Lyo NZY Reverse Transcriptase
Lyo NZY Reverse Transcriptase, provided freeze-dried, synthesizes cDNA strand in the presence of a primer using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50°C).
Lyo NZY Reverse Transcriptase is a freeze-dried enzyme provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. Lyo NZY Reverse Transcriptase is a modified recombinant form of the Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase purifiedfrom Escherichia coli. Lyo NZY Reverse Transcriptase synthesizes the complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template in a wide range of temperatures (37-50°C). High reaction temperatures are beneficial to improve specificity and to allow synthesis of cDNA from complex templates, including those with high GC-content or with high degree of secondary structure. The enzyme lacks 3´→5´ exonuclease activity and has no RNase H activity, enabling improved synthesis of full-length cDNA even for long mRNA, using random priming. Thus, Lyo NZY Reverse Transcriptase gives high yields of cDNA up to 7 kb. Lyo NZY Reverse Transcriptase can be used in first-strand cDNA synthesis experiments, RT-PCR, RT-qPCR, DNA labelling and analysis of RNA by primer extension.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | cDNA synthesis, RT-PCR, RT-qPCR |
Features | Fast, High yield, Thermostable |
Format | Enzyme/Protein, Lyophilized |
Product Category | cDNA Synthesis |
Reaction Temperature | 50 °C |
Heat Inactivation | 85 °C |
Protocol time | Approx. 60 min |
– Lyo NZY Reverse Transcriptase (100000 U)
– Lyo NZY-RT resuspension buffer
– Reaction Buffer (10x)
– 0.1 M DTT
– Lyo NZY-RT resuspension buffer
– Reaction Buffer (10x)
– 0.1 M DTT
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | cDNA synthesis, RT-PCR, RT-qPCR |
Features | Fast, High yield, Thermostable |
Format | Enzyme/Protein, Lyophilized |
Product Category | cDNA Synthesis |
Reaction Temperature | 50 °C |
Heat Inactivation | 85 °C |
Protocol time | Approx. 60 min |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | cDNA synthesis, RT-PCR, RT-qPCR |
Features | Fast, High yield, Thermostable |
Format | Enzyme/Protein, Lyophilized |
Product Category | cDNA Synthesis |
Reaction Temperature | 50 °C |
Heat Inactivation | 85 °C |
Protocol time | Approx. 60 min |
CoA
Certificate of Analysis