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DpnI

Storage Conditions:
-30 °C to -15 °C
DpnI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnI gene from  Diplococcus pneumoniae. DpnI activity is blocked by overlapping CpG methylation. NZYtech’s DpnI is an enzyme that acts at 37 °C.
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Dry Ice
From €24.00
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. DpnI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnI gene from Diplococcus pneumoniae. DpnI activity is blocked by overlapping CpG methylation. The optimum reaction temperature is at 37 °C.

Concentration: 10000 U/mL

Reaction Buffer: NZYtech provides DpnI with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided).

Activity in NZYtech Buffers:
NZYBuffer A: 70%
NZYBuffer B: 50%
NZYBuffer C: 100%
NZYBuffer U: 100%

Minimize exposure of enzyme to temperatures higher than -20 °C.

Neoschizomers: BfuCI, Bsp143I, BstENII, BstKTI, BstMBI, DpnII, Kzo9I, NdeII
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers MalI
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 80 °C for 20 minutes
Protocol time Approx. 60 min
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers MalI
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 80 °C for 20 minutes
Protocol time Approx. 60 min

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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