We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Only %1 left
SKU
DpnI
DpnI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnI gene from Diplococcus pneumoniae. DpnI activity is blocked by overlapping CpG methylation. NZYtech’s DpnI is an enzyme that acts at 37 °C.
Availability
Ready to Ship
Dry Ice
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences.
DpnI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnI gene from Diplococcus pneumoniae. DpnI activity is blocked by overlapping CpG methylation. The optimum reaction temperature is at 37 °C.
Concentration: 10000 U/mL
Reaction Buffer: NZYtech provides DpnI with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYtech Buffers:
NZYBuffer A: 70%
NZYBuffer B: 50%
NZYBuffer C: 100%
NZYBuffer U: 100%
Minimize exposure of enzyme to temperatures higher than -20 °C.
Neoschizomers: BfuCI, Bsp143I, BstENII, BstKTI, BstMBI, DpnII, Kzo9I, NdeII
Concentration: 10000 U/mL
Reaction Buffer: NZYtech provides DpnI with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYtech Buffers:
NZYBuffer A: 70%
NZYBuffer B: 50%
NZYBuffer C: 100%
NZYBuffer U: 100%
Minimize exposure of enzyme to temperatures higher than -20 °C.
Neoschizomers: BfuCI, Bsp143I, BstENII, BstKTI, BstMBI, DpnII, Kzo9I, NdeII
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -30 °C to -15 °C |
Application | DNA digestion |
Features | Standard |
Format | Liquid |
Isoschizomers | MalI |
Incubation Temperature | 37 °C |
Product Category | Restriction Enzymes |
Heat Inactivation | 80 °C for 20 minutes |
Protocol time | Approx. 60 min |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -30 °C to -15 °C |
Application | DNA digestion |
Features | Standard |
Format | Liquid |
Isoschizomers | MalI |
Incubation Temperature | 37 °C |
Product Category | Restriction Enzymes |
Heat Inactivation | 80 °C for 20 minutes |
Protocol time | Approx. 60 min |
CoA
Certificate of Analysis