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EcoRI

Storage Conditions:
-30 °C to -15 °C
EcoRI is a Restriction Endonuclease purified from an  Escherichia coli  strain that carries the EcoRI gene from Escherichia coli RY 13. EcoRI activity is blocked by some combinations of overlapping CpG methylation.
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Dry Ice
From €24.00
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. EcoRI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the EcoRI gene from Escherichia coli RY 13. EcoRI activity is blocked by some combinations of overlapping CpG methylation. The optimum reaction temperature is at 37 °C.

Concentration: 10000 U/mL

Reaction Buffer: NZYtech provides EcoRI with a specific buffer, termed NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).

Activity in NZYtech Buffers:
NZYBuffer A: 100%
NZYBuffer B: (120)%
NZYBuffer C: (80)%
NZYBuffer U: 100%

() weak star activity is detected

Minimize exposure of enzyme to temperatures higher than -20 °C.
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers FunII
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers FunII
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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