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BglII
BglII is a Restriction Endonuclease purified from an Escherichia coli strain that carries the BglII gene from Bacillus globigii. BglII activity is not affected by Dam methylation, Dcm methylation or CpG methylation.
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Dry Ice
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences.
BglII is a Restriction Endonuclease purified from an Escherichia coli strain that carries the BglII gene from Bacillus globigii. BglII activity is not affected by Dam methylation as well as by Dcm and CpG methylations. The optimum reaction temperature is at 37 °C.
Concentration: 10000 U/mL
Reaction Buffer: NZYtech provides BglII with a specific buffer, NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYtech Buffers:
NZYBuffer A: 100%
NZYBuffer B: (100)%
NZYBuffer C: (60)%
NZYBuffer U: 100%
() weak star activity is detected
Minimize exposure of enzyme to temperatures higher than -20 °C. "
Concentration: 10000 U/mL
Reaction Buffer: NZYtech provides BglII with a specific buffer, NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYtech Buffers:
NZYBuffer A: 100%
NZYBuffer B: (100)%
NZYBuffer C: (60)%
NZYBuffer U: 100%
() weak star activity is detected
Minimize exposure of enzyme to temperatures higher than -20 °C. "
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -30 °C to -15 °C |
Application | DNA digestion |
Features | Standard |
Format | Liquid |
Incubation Temperature | 37 °C |
Product Category | Restriction Enzymes |
Heat Inactivation | 65 °C for 20 minutes |
Protocol time | Approx. 60 min |
Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii.
Ribeiro O, Magalhães F, Aguiar TQ, Wiebe MG, Penttilä M, Domingues L.
Bioengineered, 2013
Ribeiro O, Magalhães F, Aguiar TQ, Wiebe MG, Penttilä M, Domingues L.
Bioengineered, 2013
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -30 °C to -15 °C |
Application | DNA digestion |
Features | Standard |
Format | Liquid |
Incubation Temperature | 37 °C |
Product Category | Restriction Enzymes |
Heat Inactivation | 65 °C for 20 minutes |
Protocol time | Approx. 60 min |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -30 °C to -15 °C |
Application | DNA digestion |
Features | Standard |
Format | Liquid |
Incubation Temperature | 37 °C |
Product Category | Restriction Enzymes |
Heat Inactivation | 65 °C for 20 minutes |
Protocol time | Approx. 60 min |
CoA
Certificate of Analysis