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One-step NZYSpeedy RT-qPCR Green kit, ROX plus

Storage Conditions:
-85°C to -15°C
One-step NZYSpeedy RT-qPCR Green, ROX plus kit is an optimized and efficient reaction mixture designed for cDNA synthesis and real-time PCR in a single tube. It includes a One-step NZYSpeedy qPCR Green master mix and a separate NZYRT mix for reverse transcription.
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From €112.00
One-step NZYSpeedy RT-qPCR Green kit, ROX plus is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZYSpeedy qPCR Green master mix, ROX plus developed for fast PCR, provided as a 2× reaction mixture, that contains all components necessary for real-time PCR, including a green intercalating dye, dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix which comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit enables fast and highly reproducible procedures on the most common real-time PCR apparatus from either total RNA or mRNA. It is optimized for intercalating green dye detection on different instruments. The latest developments in PCR enhancers have been incorporated in the One-step NZYSpeedy RT-qPCR Green kit, ROX plus, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results.
More Information
Shipping Conditions Dry Ice
Storage Conditions -85°C to -15°C
Starting Material RNA
Features Fast, Hot-start
Detection Method Intercalating dye
Reference Dye ROX plus
Format Kit, Liquid
Product Category Real-Time PCR
Compatibility AB™ 7000, AB™ 7300, AB™ 7700, AB™ 7900 Fast, AB™ 7900 HT, AB™ 7900 HT Fast, AB™ StepOne Plus™, AB™ StepOne™
Protocol time Approx. 60 min
Hot Start Yes
– One-step NZYSpeedy qPCR Green master mix (2x), ROX plus
– NZYRT mix
– DEPC-treated H2O
More Information
Shipping Conditions Dry Ice
Storage Conditions -85°C to -15°C
Starting Material RNA
Features Fast, Hot-start
Detection Method Intercalating dye
Reference Dye ROX plus
Format Kit, Liquid
Product Category Real-Time PCR
Compatibility AB™ 7000, AB™ 7300, AB™ 7700, AB™ 7900 Fast, AB™ 7900 HT, AB™ 7900 HT Fast, AB™ StepOne Plus™, AB™ StepOne™
Protocol time Approx. 60 min
Hot Start Yes
More Information
Shipping Conditions Dry Ice
Storage Conditions -85°C to -15°C
Starting Material RNA
Features Fast, Hot-start
Detection Method Intercalating dye
Reference Dye ROX plus
Format Kit, Liquid
Product Category Real-Time PCR
Compatibility AB™ 7000, AB™ 7300, AB™ 7700, AB™ 7900 Fast, AB™ 7900 HT, AB™ 7900 HT Fast, AB™ StepOne Plus™, AB™ StepOne™
Protocol time Approx. 60 min
Hot Start Yes

MSDS

Material Safety Data Sheets

CoA

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