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Multiplex PCR NZYTaq 2x Colourless Master Mix
Multiplex PCR NZYTaq 2× Colourless Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube.
Multiplex PCR NZYTaq 2× Colourless Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube. The Master Mix contains dNTPs and reaction buffer at optimal concentrations for efficient multiplexing of targets ranging in size from 70 bp to 2.5 kb over a broad range of primer and template concentrations. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. Amplification efficiency is further improved by the inclusion of a recombinant modified enzyme derived from Taq DNA polymerase, which was engineered to display a hot-start-like PCR capacity and high processivity (fast polymerisation). The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers, and thus ensuring highly specific and sensitive multiplex PCR amplification.
Extension time: 30 sec/kb at 72 °C
3'→5' activity: no
Product overhang: 3'-A
Extension time: 30 sec/kb at 72 °C
3'→5' activity: no
Product overhang: 3'-A
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Application | Multiplex PCR, TA Cloning, Typing/Detection |
Features | Hot-start, Multiplex |
Product Length | 0-3 kb |
Format | Mastermix |
Product Category | End-Point PCR |
Concentration | 0.2 U/μL |
Hot Start | Yes |
Multiplex PCR NZYTaq 2× Colourless Master Mix (0.2 U/μL)
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Application | Multiplex PCR, TA Cloning, Typing/Detection |
Features | Hot-start, Multiplex |
Product Length | 0-3 kb |
Format | Mastermix |
Product Category | End-Point PCR |
Concentration | 0.2 U/μL |
Hot Start | Yes |
A new multiplex PCR protocol to detect mixed trypanosomatid infections in species of Apis and Bombus
Bartolomé C, Buendía M, Benito M, De la Rúa P, Ornosa C, Martín-Hernández R, Higes M, Maside X.
J Invertebr Pathol, 2018
Bartolomé C, Buendía M, Benito M, De la Rúa P, Ornosa C, Martín-Hernández R, Higes M, Maside X.
J Invertebr Pathol, 2018
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Application | Multiplex PCR, TA Cloning, Typing/Detection |
Features | Hot-start, Multiplex |
Product Length | 0-3 kb |
Format | Mastermix |
Product Category | End-Point PCR |
Concentration | 0.2 U/μL |
Hot Start | Yes |
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Application | Multiplex PCR, TA Cloning, Typing/Detection |
Features | Hot-start, Multiplex |
Product Length | 0-3 kb |
Format | Mastermix |
Product Category | End-Point PCR |
Concentration | 0.2 U/μL |
Hot Start | Yes |
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Application | Multiplex PCR, TA Cloning, Typing/Detection |
Features | Hot-start, Multiplex |
Product Length | 0-3 kb |
Format | Mastermix |
Product Category | End-Point PCR |
Concentration | 0.2 U/μL |
Hot Start | Yes |
CoA
Certificate of Analysis