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SKU
MB00801

T4 Polynucleotide Kinase (T4 PNK)

Storage Conditions:
-85 °C to -15 °C
T4 Polynucleotide Kinase catalyses the transfer of the terminal phosphate of ATP to 5′-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3′-mononucleotides.
Availability
Ready to Ship
Dry Ice
Unit Size
500 U
€81.00
T4 Polynucleotide Kinase (T4 PNK) catalyses the transfer of the terminal phosphate of ATP to 5'-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3'-mononucleotides. This enzyme also possesses a 3'-phosphatase and 2', 3' cyclic phosphodiesterase activity. The enzyme can be used for the following applications: 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation; end-labelling DNA or RNA for use as probes, for DNA sequencing or for DNA-protein foot printing; removal of 3' phosphoryl groups.
Applications:
– End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting
– 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation
– Removal of 3´-phosphoryl groups
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3'-dephosphorylation, 5´-phosphorylation
Product Category Other Modifying Enzymes
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 500 U
– T4 Polynucleotide Kinase (500 U)
– Reaction buffer (10x)
– Dilution buffer
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3'-dephosphorylation, 5´-phosphorylation
Product Category Other Modifying Enzymes
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 500 U
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3'-dephosphorylation, 5´-phosphorylation
Product Category Other Modifying Enzymes
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 500 U

MSDS

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CoA

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